Detection of genes mutations in the K-ras, H-ras and EGFR in samples of blood plasma and cervical smears for patients with cervical intraepithelial neoplasia III and cervical cancer
نویسنده
چکیده
Introduction: Cervical cancer is the second most important cancer in women worldwide, and the second cause of cancer death in women. It has been shown that the process of cervical carcinogenesis presents as genetic and epigenetic components as environmental issues. At present, many studies are addressed in searching for molecular markers such as mutations in oncogenes and/or tumor suppressor genes that are associated with the progression of this disease, the most studied candidate genes in cervical cancer in different populations have been H-ras, K-ras, EGFR among others. Objective: The present study identified human papilloma virus (HPV) generic and specific in DNA-free plasma and cervical smears of invasive cervical cancer patients and patients with cervical intraepithelial neoplasia (CIN) III in addition to assessing genetic alterations, such as mutations in the genes H-ras, EGFR and K-ras. Methods: To do so generic HPV was detected by PCR with primers GP5+/GP6+, and specific HPV 16 and 18 in E6/E7 region; to detect mutations in codon 12 of H-ras, codons 12 and 13 of K-ras and EGFR exon 21 was conducted by direct sequencing of PCR products of these gene fragments. Results: Getting a good correlation between samples of blood plasma and cervical smears for both; the findings of HPV p=0.0374 and evaluated mutations p=0. In general, for EGFR in exon 21 mutations were not found, as for codons 12 and 13 in K-ras and codon 12 in H-ras. Conclusion: The use of DNA in plasma may be relevant to the analysis of mutations and the presences of tumor markers are not available from other samples.
منابع مشابه
Prevalence Of Human Papilloma Virus Among Women With Cervical Intraepithelial Neoplasia III And Invasive Cervical Cancer From 2001 To 2006 In Bandarabas
Background and Objective: To estimate the risk of human papilloma virus (HPV) infection for cervical malignancies, we conducted a case-control study in southern Iran (Hormozgan province). Materials and Methods: For this purpose,52 paraffin embedded blocks with exact diagnosis of cervical carcinoma(50 carcinomas and 2 carcinomas in situ) from 2001 to 2006 and 52 praffin embedded blocks of ce...
متن کاملمقایسه حضور فیبروسیت های CD34 مثبت دراسترومای مجاور اینترااپیتلیال نئوپلازی با درجه بدخیمی بالاو کارسینوم مهاجم سلول سنگفرشی سرویکس رحم
Background: Cervical cancer does not have symptoms in the early stages and almost all invasive carcinomas of the cervix have an earlier stage in which abnormal cells are confined to the epithelium. The intra-epithelial lesions associate with cytological features of invasive cancer. So, it is important to differentiate a cervical intraepithelial neoplasia (CIN) Ш from an invasive cervical squamo...
متن کاملComparing the effect of the order of cell Collection for Pap Smear on the results of cytologic Diagnosis
Background: World wide, Cervical cancer comprises 12% of all cancers in women. In developed countries, routine cervical cytology tests resulted in a large decline in cervical cancer incidence. This study is a descriptive- analytical research to evaluate the order of cell sampling from exocervix and endocervix and compare final cytological diagnosis. Materials and methods: Totally, 1129 smears ...
متن کاملPrevalence of K-RAS mutations and CA125 tumor marker in patients with ovarian carcinoma
Background: Ovarian carcinoma is one of the leading causes of cancer-related death among females. K-ras codon 12 mutations are commonly occurring mutations in different types of cancers and leads to resistance against anti-EGFR therapeutics. Hence, determination of mutations in k-ras gene is crucial for predicting response to anti-EGFR therapies. This study aimed to evaluate the prevalence of k...
متن کاملMolecular Identification of Human Papiloma Virus (HPV) in Cervical Neoplasia and the Risk Factors Affecting Cervical Cancer in Isfahan
The rate of cervical cancer in Asians is deferent, based on the risk factors affecting and the rate of papiloma virus infection among females. Cervical cancer is one of the important cancers between Iranian women. In the present study the rate of cervical neoplasia was detected in Isfahan and some risk factors affecting the incidence of cervical cancer was analyzed. The human papiloma virus inf...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2012